Download The Grass Widow's Tale (Felse, Book 7) by Ellis Peters PDF

By Ellis Peters

Whilst George Felse unearths himself referred to as away to London on pressing police company, his spouse Bunty is left on my own feeling depressed at the eve of her forty first birthday. To shake off her black temper she is going out to the neighborhood pub the place an opportunity assembly areas her in lethal possibility.

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Popovi~,M. Sarngadharan,E. Read, and R. C. Gallo,Science 224, 497 (1984). 12M. Mackett,G. L. Smith,and B. Moss, in "DNA Cloning"(D. M. ), Vol. 2, p. 191. IRL Press, Oxford, 1985. ~3p. Earl and B. Moss, in "CurrentProtocolsin MolecularBiology"(F. M. ), Vol. 2, suppl. 15, pp. 16. Wiley,New York. 14 VIRUS AND VIRAL PROTEIN MEDIATED CELL-CELL FUSION [2] control of a bacteriophage T7 promoter is constructed. ~4 Expression of CD4 is achieved on coinfection of cells with the latter virus, as well as a second recombinant vaccinia virus that contains the bacteriophage T7 RNA polymerase gene under control of a vaccinia virus promoter.

Wong-Staal, and G. Franchini, Science 244, 694 (1989). s y. Sakai'and H. Shibuta, J. Virol. 63, 3661 (1989). 6 M. Whitt, P. Zagouras, B. Cruse, and J. K. Rose, J. Virol. 64, 4907 (1990). ~ t © 1993by AcademicPr--~,Inc. METHODSIN ENZYMOLOGY,VOL. 221 Allrightsofrel~'oduclionin any formreserved. [2] VACCINIA IN STUDY OF HIV-MEDIATED MEMBRANE FUSION 13 Recombinant vaccinia virus technology has been used to study membrane fusion mediated by HIV-1 envelope glycoprotein (Env) and its cellular receptor CD4.

Y. ), Vol. 2, p. 39. Academic Press, New York, 1982. 57 y. Okada and F. Murayama, Exp. Cell Res. 44, 527 (1966). 5s T. Ohno-Shosaku and Y. Okada, J. Membr. Biol. 85, 269 (1985). 100 1000- / "O O k. -. d O Z 5 Z / 0 0-"" 0 i(~,, © | 5 Time | I 10 of t I I .... I addition I 30(min) 15 of EDTA FIO. 6. Appearance of the stage requiring calcium ions. At appropriate times during incubation of the EAT cell-HVJ complex at 37 °, calcium ions are removed by the addition of ethylenediaminetetmacetic acid (EDTA) and, after incubation for 60 min at 37 °, the cells are observed.

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