Download Renal and Urinary Proteomics: Methods and Protocols by Thongboonkerd PDF

By Thongboonkerd

In line with study and effects from the foreign Human Kidney and Urine Proteome undertaking, this guide and reference offers present tools and protocols for the research of human kidney and urine tract. The world's prime specialists hide every thing from easy innovations for regimen research to complex tools for tricky samples or analytes. Written with the practitioner in brain, all of the equipment and strategies are defined with step by step protocols subsidized through necessary functional suggestion taken from genuine existence experiments.

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1996) Osmotic regulation of sorbitol in the thick ascending limb of Henle’s loop. Am J Physiol, 270, F275–F282. Kinne-Saffran, E. K. (1997) Sorbitol uptake in plasma membrane vesicles isolated from immortalized rabbit TALH cells: activation by a Ca2+/calmodulin-dependent protein kinase. J Membr Biol, 159, 231–238. , Tohyama, M. et al. (1998) Inhibition of myo-inositol transport causes acute renal failure with selective medullary injury in the rat. Kidney Int, 53, 146–153. L. A. (1996) Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats.

Right panel: quantitative analysis of fluorescence intensity in fura-2-stained TALH cells after osmotic stress treatment. family: hsc73 and HSP8a. 8b) in TALH-NaCl cells [30]. HSPs enhance survival of the stressed cells by acting as molecular chaperones when normal cellular protein synthesis is inhibited through denaturation. Owing to recent investigations on both renal and nonrenal cells, similar effects of osmotic stress concerning the expression of α-crystallin B chain and HSP70 under stress situation have been reported [53–55].

1998) Inhibition of myo-inositol transport causes acute renal failure with selective medullary injury in the rat. Kidney Int, 53, 146–153. L. A. (1996) Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats. Kidney Int, 50, 1202–1211. , Noguchi, T. et al. (1994) In vivo osmoregulation of Na/myo-inositol cotransporter mRNA in rat kidney medulla. J Am Soc Nephrol, 5, 62–67. W. and Eckstein, A. (1995) Osmotic regulation of the betaine metabolism in immortalized renal cells.

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