Download NMR-Bioassay Guided Isolation of the Natural 20S Proteasome by Martin Lorenz Stein PDF

By Martin Lorenz Stein

Martin Stein's thesis describes a unique method for ordinary product discovery. because of its excessive measure of reproducibility, robustness and sensitivity, the process can be used to observe even hint quantities of bioactive ingredients in heterogeneous matrices equivalent to fermentation broths or crude natural extracts. This examine is therefore suitable for quite a few researchers operating in ordinary product discovery. purposes of this novel NMR-based strategy comprise appropriate environmental triggers for the induction of biosynthetic machineries. the writer demonstrates the intense worth of this procedure through the winning isolation of 2 powerful inhibitors of the pharmaceutically appropriate proteasome middle particle from the insect pathogen photorhabdus luminescens. This thesis has ended in a couple of guides in high-impact journals.

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NMR-Bioassay Guided Isolation of the Natural 20S Proteasome Inhibitors from Photorhabdus Luminescens: A Novel NMR-Tool for Natural Product Detection

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Peptides were identified by ESI-MS measurements. The main hydrolysis site is located after phenylalanine, whereas cleavage after tryptophan occurs only to a minor proportion of approximately 5 % In order to investigate the contribution of the different proteolytic activities, the digestion was assessed with inactivated T1A mutants of both the β2 and β5 subunits. Whereas β5 harbors an intrinsic specificity for hydrophobic residues, β2 has been shown to accept a broad range of suitable substrates ranging from basic to branched hydrophobic residues.

Stein, P. Beck, M. Kaiser, R. F. Becker, M. Groll, Proc. Natl. Acad. Sci. A. J. Crawford, B. C. E. Irvine, Leuk. Res. E. J. S. B. Ojeda, Comb. Chem. H. Haverkamp, D. Schouten, M. Doeleman, U. Wollenzien, J. J. Stal, Isme J. P. Thawornwiriyanun, S. Tanasupawat, C. Dechsakulwatana, S. Techkarnjanaruk, W. Suntornsuk, Appl. Biochem. Biotechnol. A. Khanafari, D. Khavarinejad, A. Y. Tu, C. Jeffries, H. Ruan, C. Nelson, D. A. M. C. P. Hester, T. A. Khan, L. Walker, K. Guy, B. Yan, J. nat. prod. E.

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