By P. Narayanasamy
Experiences at the phenomenon of plant pathogenesis (disease improvement) were invaluable to have a deep perception into the interactions among host plant and the pathogen. looking on the degrees of susceptibility (compatibility) or resistance (incompatibility) of the host plant and virulence of the pathogen, affliction improvement may perhaps growth, both resulting in symptom expression or bring about the suppression of pathogen proliferation. Molecular recommendations were utilized to clarify the character of interactions among the gene items of the plant and pathogen at mobile and molecular degrees. profitable evasion of host s surveillance process and next actions of metabolites of the pathogen (enzymes and pollutants) encoded by means of pathogen genes counteracting the consequences of varied defense-related antimicrobial compounds current already or produced via the host vegetation, after initiation of an infection were seriously studied via utilising a variety of molecular strategies. as well as learning a number of stages of disorder improvement in person crops, molecular tools were verified to be potent, in collecting info on numerous elements of epidemiology less than typical stipulations the place the interplay of pathogen with populations of crops is stimulated considerably by way of the environmental stipulations current in several ecosystems. This quantity makes a speciality of the potential of employing the data on pathogenesis and molecular epidemiology to figure out the weak levels within the existence cycles of the pathogens that may be disrupted to accomplish better illness keep watch over.
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Extra info for Molecular Biology in Plant Pathogenesis and Disease Management: Disease Development, Volume 2
The AB-toxin production by A. brassicola was studied in detail. The spore germination fluids (SGFs) contained AB-toxin as revealed by leaf necrosis assay. The AB-toxin was released in SGFs incubated for 12 h on host leaves and the toxin production increased with increase in incubation period. The SGFs were concentrated 10-fold and analyzed by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) technique. One protein band (38 kDa) was detected in the SGF supplied with active fraction.
The G (alpha) subunit BCG1 has been shown to have an important role during infection of host plants by B. cinerea. The mutants (Δbcg1) could conidiate, penetrate host leaves and induce small primary lesions. However, further development of symptoms was arrested in contrast to the uninhibited disease development in infection by wild-type. The fungal genes, whose expression on the host plant was specifically affected in bcg1 mutants, were identified by employing suppression subtractive hybridization (SSH).
In addition, entry into the host by direct penetration by the wild type, but not by the mutant, was visualized by scanning electron microscopy (SEM). However, demonstration of the requirement of cutinase gene for pathogenicity by complementation of the mutant with the Pbc1 leading to restoration of pathogenicity, will provide strong support to conclusion on the requirement of cutinase for penetration of cuticle (Li et al. 2003). The role of cutinase as a pathogenicity factor may be important in certain pathosystems, but many pathogens may produce different enzymes acting synergisticaly making the presence of cutinase as not a critical factor for pathogenicity.