By Nejat Duzgunes (Eds.)
The significantly acclaimed laboratory normal, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. considering the fact that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct today--truly an important booklet for researchers in all fields of existence sciences.
* significant themes lined include:
* Cell-cell fusion mediated through viruses and viral proteins
* Conformational adjustments of proteins in the course of membrane fusion
* Membrane fusion in the course of exocytosis
* Intracellular membrane fusion
* Membrane fusion in fertilization
* creation of macromolecules into cells through membrane fusion
* Protoplast fusion
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Extra resources for Membrane Fusion Techniques Part B
Popovi~,M. Sarngadharan,E. Read, and R. C. Gallo,Science 224, 497 (1984). 12M. Mackett,G. L. Smith,and B. Moss, in "DNA Cloning"(D. M. ), Vol. 2, p. 191. IRL Press, Oxford, 1985. ~3p. Earl and B. Moss, in "CurrentProtocolsin MolecularBiology"(F. M. ), Vol. 2, suppl. 15, pp. 16. Wiley,New York. 14 VIRUS AND VIRAL PROTEIN MEDIATED CELL-CELL FUSION  control of a bacteriophage T7 promoter is constructed. ~4 Expression of CD4 is achieved on coinfection of cells with the latter virus, as well as a second recombinant vaccinia virus that contains the bacteriophage T7 RNA polymerase gene under control of a vaccinia virus promoter.
Wong-Staal, and G. Franchini, Science 244, 694 (1989). s y. Sakai'and H. Shibuta, J. Virol. 63, 3661 (1989). 6 M. Whitt, P. Zagouras, B. Cruse, and J. K. Rose, J. Virol. 64, 4907 (1990). ~ t © 1993by AcademicPr--~,Inc. METHODSIN ENZYMOLOGY,VOL. 221 Allrightsofrel~'oduclionin any formreserved.  VACCINIA IN STUDY OF HIV-MEDIATED MEMBRANE FUSION 13 Recombinant vaccinia virus technology has been used to study membrane fusion mediated by HIV-1 envelope glycoprotein (Env) and its cellular receptor CD4.
Y. ), Vol. 2, p. 39. Academic Press, New York, 1982. 57 y. Okada and F. Murayama, Exp. Cell Res. 44, 527 (1966). 5s T. Ohno-Shosaku and Y. Okada, J. Membr. Biol. 85, 269 (1985). 100 1000- / "O O k. -. d O Z 5 Z / 0 0-"" 0 i(~,, © | 5 Time | I 10 of t I I .... I addition I 30(min) 15 of EDTA FIO. 6. Appearance of the stage requiring calcium ions. At appropriate times during incubation of the EAT cell-HVJ complex at 37 °, calcium ions are removed by the addition of ethylenediaminetetmacetic acid (EDTA) and, after incubation for 60 min at 37 °, the cells are observed.