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By Bernard L Horecker; et al

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And Burris, R. H. (1972). Biochim. Biophys. Ada 275, 93-101. 10. Kurtz, D. , McMillan, R. , Burgess, B. , Mortenson, L. , and Holm, R. H. (1979). Proc. Natl. Acad. Sei. A. 76, 4986-4989. 11. Shah, V. , and Brill, W. J. (1977). Proc. Natl. Acad. Sei. A. 74, 3249-3253. 12. Simpson, F. , and Burris, R. H. (1984). Science 224, 1095-1097. 13. , Kamen, M. , and Bregoff, H. M. (1950). J. Biol. Chem. 182, 153-170. 14. Ormerod, J. , and Gest, H. (1962). Bacteriol. Rev. 26, 51-66. 15. Bulen, W. , and Le Comte, J.

D Reported previously. 32 PAUL W. LUDDEN AND GARY P. ROBERTS effect on activity. Likewise, derivatization of adenine to its etheno analog does not affect the Km or Vmax of the enzyme. The compound iVa-dansyl-iVw-(l,iV6-etheno-ADP-ribosyl)arginine methyl ester (Fig. 2) provides a very effective assay for DRAG (39). There is fluorescence quenching between the ethenoadenine and dansyl groups of this compound and, upon hydrolysis by DRAG or phosphodiesterase, the quenching is relieved and fluorescence enhancement is observed.

1982). Biochem. J. 203,663-668. 57. Gotto, J. , and Yoch, D. C. (1982). J. Biol. Chem. 257, 2868-2873. 58. Preston, G. , and Ludden, P. W. (1982). Biochem. J. 205, 489-494. 59. Zumft, W. , and Castillo, F. (1978). Arch. Microbiol. 117, 53-60. 60. Carithers, R. , Yoch, D. C, and Arnon, D. I. (1979). J. Bacteriol. 137, 779-789. 61. Triplett, E. , Wall, J. , and Ludden, P. W. (1982). J. Bacteriol. 152, 786-791. 62. Weare, N. , and Shanmugam, K. T. (1976). Arch. Microbiol. 110, 207-213. 63. , and Vignais, P.

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