By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Ira H. Pastan
The severely acclaimed laboratory common, equipment in Enzymology, is among the so much hugely revered courses within the box of biochemistry. due to the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - actually a necessary booklet for researchers in all fields of lifestyles sciences.Key positive aspects* Covers fundamentals in nice element* Laboratory specifications and Media phone CultureTechniques: Monolayers, progress size, and Mass tradition
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Sample text
After all items have been removed from the cabinet, the cabinet should be allowed to operate for a few minutes to assure the removal of airborne contaminants. The work surface should then be decontaminated with a chemical disinfectant. 14 In addition to emphasizing the importance of good laboratory practice, the guidelines encourage the creation of biosafety committees to oversee work involving human cell lines, assign responsibilities to the laboratory supervisor for preparing safety protocols, recommend certain medical surveillance and screening procedures, and limit access to the cell culture laboratory to authorized individuals.
Dirnmick, W. Vogl, and M. A. Chatigny, in "Biohazards in Biological Research" (A. Hellman, M. N. Oxman, and R. ), p. 246. , Cold Spring Harbor, New York, 1973. s A. G. Wedum, Public Health Rep. 79, 619 (1964). a G. B. Phillips, J. Chem. Educ. 42, Part One, A43; ibid. Part Two, All7 (1965). 10 H. M. " 1, 169 (1969). 11 M. A. Chatigny and D. I. Clinger, in "An Introduction to Experimental Aerobiology" (L. Dimmick and A. B. ), p. 194. Wiley, New York, 1969. ~2 M. A. Chatigny, Adv. Appl. Microbiol.
3. , Cold Spring Harbor, New York, 1973. 2 G. J. Todaro, in "Biohazards in Biological Research" (A. Hellman, M. N. Oxman, and R. ), p. 114. , Cold Spring Harbor, New York, 1973. 3 j. A. Schneider, in "Biohazards in Biological Research" (A. Hellman, M. N. Oxman, and R. ), p. 191. , Cold Spring Harbor, New York, 1973. 38 BASIC METHODS [4] established. W h e r e infectious viruses are not intentionally handled, an assumption of hazard should be the basis for establishing safeguards. Potential for Exposure Analysis of c o m p r e h e n s i v e surveys of laboratory-acquired infections reveal that recognized accidents are not the cause for m o s t infections a m o n g l a b o r a t o r y workers° 4-6 Indeed, f e w e r than 20% of d o c u m e n t e d infections can be attributed to accidental contact, ingestion, or injection with infectious materials.