Download Advances in Biomedical Engineering. Volume 4 by J.H.U. Brown, J.F. Dickson PDF

By J.H.U. Brown, J.F. Dickson

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T h e initial description of this computer-based method was given by Hites and B i e m a n n (1970), and t h e term "mass c h r o m a t o g r a p h y " was used to describe the process. This form of selective ion detection requires a G C - M S - C O M system r a t h e r t h a n a G C - M S assembly, b u t the uses of t h e method lie in capabilities for detecting specific compounds with relatively high sensitivity in detection. An a d v a n t a g e of this procedure is t h a t numerous programmed analyses can be carried out for a single experimental analytical run.

A small computer system can be used in on-line fashion with a highresolution mass spectrometer (resolution about 1:10,000) ; an example of a suitable system is t h a t described by Klimowski et al. (1970). T h e problems involved in systems of this kind are discussed in a recent review ( V e n k a t r a r a g h a v a n et al, 1970). Low-resolution techniques generally involve either repetitive scanning, using 2 - 3 seconds for a full mass scan, or selective ion detection for a single ion or a few ions.

Analytical studies of h u m a n metabolites ranging from u r i n a r y volatiles to steroids are often best carried out by gas-phase methods. I t is therefore difficult t o summarize current a n d possible future applications with a n y sense of completeness.

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