By Richard Twyman
This publication emphasises the unifying ideas and mechanisms of molecular biology, with widespread use of tables and containers to summarise experimental information and gene and protein capabilities.
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Extra info for Advanced molecular biology: a concise reference
11:464-470. M. (1996) Polygenic disease - methods for mappingcomplex disease traits. Trends Genet. 11:513-519. M. (1994) The molecular basis of genetic dominance. J. Med. Genet. 31: 89-98. (1995) Thegenetic contribution tophenotype. Hum. Genet. 9 5 127-148. This Page Intentionally Left Blank Chapter 2 The Cell Cycle Fundamental concepts and definitions The cell cycle is the sequence of events between successive cell divisions. g. g. cell division). Cell division must be coordinated with growth DNA andreplication so that cell size and DNA content remain constant.
The activity of MPF resides in the catalytic kinase subunit but it is dependent upon the cyclin subunit, which introduces a conformational change in its partner to stimulate kinase activity. The cell cycle kinases are thus described as cyclin-dependent kinases (CDKs) and function as CDKcyclin holoenzymes. This strategy of cell cycle regulation appears to be conserved throughout the eukaryotes. CDK-cyclin diversity in the yeast and animal cellcycles. A number of potential CD& have been isolated from yeast, but only CDC28 in S.
The proteins form dimers, and competition between them, 34 Advanced Molecular Biology reflecting their relative abundances, determines the fate of the cell. The effectorsof BCL-2 signaling are a family of cysteine proteases related to the interleukin-lp converting enzyme (ICE proteases). A number of such enzymes are synthesized by mammalian cells as inactive zymogens and are activated in a proteolytic cascade, culminating in the activation of proteins such as poly(ADP)ribose polymerase and nuclear lamins, the direct effectors of apoptotic cell behavior.